Figure 6.
Use of the pumping lid for loading of SlipChip devices. (A) Photographs of a 6-year-old volunteer with minimal training using the pumping lid to load a SlipChip device. The sample is placed in the cup at the device inlet (A1), the pumping lid is placed on the cup, and when the lid is pushed, positive pressure is generated and sample pumping starts (A2). Once the sample loading is complete, slipping two plates generates discrete compartments (A3). A video of this experiment is provided.30 (B) SlipChip sample loading by negative pressure. (B1) Schematic outline of the steps. The lid is pre-placed on the cup, and the sample is placed at a separate inlet in the device. Pulling the lid creates negative gauge pressure and initiates loading. Dead-end filling ensures that the loading stops once the device is completely filled. (B2) Photograph of a multivolume SlipChip device for digital nucleic acid quantification loaded with negative pressure pumping lid method.