Figure 5.

Abundance of φPDS1 in the fermenter vessel with and without selective conditions showing that the selective conditions greatly aided φPDS1 propagation. (a) Relative abundance of φPDS1 with and without antibiotics obtained from the total viral reads sequenced. (b) Absolute quantification of φPDS1 via qPCR targeting a segment of the phage DNA polymerase. Titre determined in copies/ml using the standard curve method. Error bars represent standard deviation (n = 3). * indicates statistically significant differences between vessels, with a p-value <0.05 after performing an unpaired t-test or the Mann – Whitney U test, depending on the data distribution (normal or non-normal, respectively).