Figure 7.

Biological characterization of φPDS1. (a) φPDS1 one-step growth curve. Sampling was performed every 15 minutes over 3 hours. The latent period was 90 minutes, and the burst size was ~ 23 copies per infected cell. (b) The titer of φPDS1 following continuous co-culture on the host over 10 days showing the persistence of the phage with its host. (c) Liquid propagation of φPDS1 on two commercial P. distasonis strains (DSM 29491 and DSM 20701) and O. splanchnicus (DSM 20712) was examined over five days with host P. distasonis APCS2/PD used as a control. Titres were determined in copies/ml via qPCR for all the experiments. Arrows on the y-axis indicate phage titer, after dilution, on initiation of the experiment. Error bars indicate standard deviation (n = 3).