Figure 1.

RBM4 could inhibit EBOV replication in both HEK293T and Huh-7 cells. (A–B) HEK293T cells and Huh-7 cells were transfected with pCAGGS-RBM4-FLAG and empty vector. Twenty-four hours later, cells were transfected with plasmids encoding NP, VP35, VP30, L and Tim-1. At 24 h post-transfection (h p.t.), medium was discarded, and cells were infected with EBOV-trVLPs. At 12 h post-infection (h p.i.), supernatants were discarded and replaced with fresh medium. At 48 h p.i., expression of RBM4 in cells was detected by WB, and the viral replication was measured by dual-luciferase assay. (C) Homology analysis of RBM4 in Ebola virus susceptible hosts. (D–E) HEK293T cells and Huh-7 cells were transfected with siRNA NC and si345. Twenty-four hours later, EBOV-trVLPs assay were performed as described in (A–B). At 48 h p.i., expression of RBM4 in cells was detected by WB, and the viral replication was measured by dual-luciferase assay. (F) EBOV-trVLPs assay were conducted with RBM4-KO and control LentiV2 cell lines. At 48 h p.i., expression of RBM4 in cells was detected by WB, and the viral replication was measured by dual-luciferase assay. The mean and SEM from one representative experiment (n = 3) of 3 independent experiments are indicated. *P < 0.05, ***P < 0.001, ****P < 0.0001 (two-tailed Student t-test).