Figure 3.

RBM4 interacts with EBOV genomic RNA instead of viral proteins. (A–B) RBM4-FLAG interacted with EBOV genome. HEK293T cells were transfected with RBM4-FLAG. Twenty-four hours later, EBOV-trVLPs assay were performed. Seventy-two hours later, expression of RBM4-FLAG and viral genome in cells were detected by WB and PCR, respectively. Then, the interaction between RBM4-FLAG and viral genome was detected using RIP assay. (C) C-terminal FLAG-tagged RBM4 no interacted with other viral proteins. Plasmids encoding VP35-HA, VP24-HA, VP30-HA, VP40-HA, NP-HA, and GP-HA were co-transfected separately with RBM4-FLAG into HEK293T cells. At 48 h p.t., cells were collected, followed by anti-FLAG Co-IP assay and WB. The mean and SEM from one representative experiment (n = 3) of 3 independent experiments are indicated. ****P < 0.0001 (two-tailed Student t-test).