Figure 6.

The N-terminal region RRM1 of RBM4 is responsible for inhibiting the replication of EBOV. (A) The plasmids vector, and encoding RBM4-FLAG, RBM4a-FLAG and RBM4b-FLAG were transfected with HEK293T cells. At 24 h p.t., EBOV-trVLPs assay were performed. At 72 h p.t., expression of RBM4 or its mutants in cells was detected by WB, and the viral replication was measured by dual-luciferase assay. (B) The plasmids vector, and encoding RBM4-FLAG, delRRM1-FLAG and delRRM2-FLAG were transfected with HEK293T cells. At 24 h p.t., EBOV-trVLPs assay were performed. At 72 h p.t., expression of RBM4 or its mutants in cells was detected by WB, and the viral replication was measured by dual-luciferase assay. (C) The plasmids vector, and encoding RBM4-FLAG, RBM4a-FLAG, RBM4b-FLAG, delRRM1-FLAG, and delRRM2-FLAG were transfected into HEK293T cells. At 24 h p.t., EBOV-trVLPs assay were performed. At 72 h p.t., cells were collected to detect mRNA level by quantitative RT-PCR. (D) Homology analysis of RRM1 of RBM4 in EBOV susceptible hosts. The mean and SEM from one representative experiment (n = 3) of 3 independent experiments are indicated. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student t-test). NS, not significant.