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Cellular vitality of organoids is evaluated by SA-β-Gal staining. (A) Cells that are not stained are defined as negative (0+); (B) Cells stained as light green are defined as 1+; (C) Cells stained as dark green are defined as 2+. Scale bar 30 μm. SA-β-Gal, senescence-associated β-galactosidase.
