Extended Data Fig. 3. Sparsely expressing mice for analysis of opsin negative cells.

a: Representative image from one of 6 FOVs from a SepW1-Cre mouse with AAV CAG-DIO-ChroME2. Scale Bar 100 µm. Inset, example images of opsin negative (above) and opsin positive (below) cell. Scale Bar (10 µm). Green is from GCaMP, red from nuclear localized mRuby3 part of the opsin construct. b: The intensity of the red fluorophore detected for each cell in one field of view (representative of all 6). Top 80^th percentile categorized as opsin positive, bottom 30^th percentile opsin negative (Note: red counts are integers and more than 30% of cells may have the same or lower 30^th percentile score). c: Evoked ΔF/F responses in non-targeted cells to 10 cell ensemble stimulation as a function of distance from the closest target, separated by opsin positive and opsin negative cells. Exclusion zone (15 µm) marked in gray. N = 57 ensembles, 6 FOVs, 4 Mice.