Skip to main content
. 2024 Jan 8;14:845. doi: 10.1038/s41598-023-50877-9

Figure 1.

Figure 1

ECAR, OCR, and ATP production rate increase acutely at 24-h following radiation in salivary acinar cells. (a) Primary acinar cells were isolated from parotid salivary glands of untreated female FVB mice (n = 3/group), cultured for 5 days, and then irradiated at 24 and 48 h prior to the seahorse XF real-time ATP rate assay. (b) Individual ECAR readings for untreated (UT), 24-h IR (24 h IR), and 48-h IR (48 h IR) groups with the responses to 2.0 μM oligomycin (oligo) injection and 0.5 μM rotenone/antimycin A (rot/AA) injection shown. (c) Average basal, oligo, and rot/AA ECAR readings for UT, 24 h IR, and 48 h IR. (d) Individual OCR readings for UT, 24 h IR, and 48 h IR groups with the responses to 2.0 μM oligomycin (oligo) injection and 0.5 μM rotenone/antimycin A (rot/AA) injection shown. (e) Average basal, oligo, and rot/AA OCR readings for UT, 24 h IR, and 48 h IR. (f) Basal ATP production rates of UT, 24 h IR, and 48 h IR groups. (g) The proportion of mitochondrial (mito) ATP production rate and glycolytic (glyco) ATP production rate for UT, 24 h IR, and 48 h IR groups. Each panel is representative of 3 independent assays (n = 3/group). Significant differences were determined using one-way ANOVA and Bonferroni post-hoc test, p < 0.05. Treatment groups with the same letter are not significantly different from each other.