Figure 5.
miR-22/Twist1 regulates MG63 migration and invasion. (A) Transwell migration assay detected the migration of MG63 transfected with both miR-22 mimic and Twist1 vector. (B) Transwell invasion assay detected the invasion of MG63 transfected with both miR-22 mimic and Twist1 vector. (C) Wound healing assay. (D) RT-qPCR detected the expression of MMP2. (E) Western blotting detected the expression of MMP2 (n = 3,*P ≤ 0.05 vs. control).