Fig. 4. The C. parvum CpGT1 and CpGT2 transporters salvage glucose phosphate.

a Solid media complementation of a mutant E. coli line lacking the glucose phosphate transport (∆uhpT). Cells were grown in M9 minimal media (M9 MM) supplemented with 10 mM glucose or glucose-6-phosphate (G6P). The indicated dilutions were plated and grown at 37 °C for 40 h before imaging. The experiment was performed three times with similar results. b Solid media complementation of the E. coli the glucose transport mutant (∆ptsG, ∆galp, ∆ptsM) grown in M9 MM supplemented with 10 mM glucose or G6P. The indicated dilutions were plated and grown at 37 °C for 40 h before imaging. The experiment was performed three times with similar results. See also Supplementary Fig. 8 for the growth assay of WT E. coli complementing with different genes. Source data are provided as an accompanying Source Data file.