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. 2024 Jan 8;14:828. doi: 10.1038/s41598-023-50667-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Knock-out validation of commercial C9orf72 antibodies indicated for Western blot. Immunofluorescent Western blot images showing nine commercial C9orf72 antibodies tested at concentrations recommended by the manufacturer. A band of ~ 52 kDa corresponding to C9orf72 (in green) was present in 2-week-old iPSC-derived motor neurons from a C9orf72 ALS/FTD patient, but absent in the KO line only for antibodies GTX632041, GTX634482, 2575-1-AP and B01-5F2. C9 = Pooled technical replicates (wells) of 2-week-old C9 ALS/FTD patient iPSC-derived motor neurons. KO = Pooled technical replicates (wells) of 2-week-old homozygous KO iPSC-derived motor neurons. Membranes were co-incubated with beta-actin loading control (42 kDa, in red).