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. 2024 Jan 8;7:62. doi: 10.1038/s42003-023-05744-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–e Plots of the five TAP properties for the κ (seagreen) and λ (plum) CSTs, and (f) the trend of the Patches of Surface Hydrophobicity (PSH) score with L_tot, using ABodyBuilder2 for structural modeling (ABB2). Amber thresholds are set based on the 5th and/or 95th percentile values of the combined set of kappa and lambda CSTs. Percentage values reflect the proportions of the correspondingly coloured light-chain class of antibody within the amber-flagged region of each distribution.