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. 2024 Jan 8;7:62. doi: 10.1038/s42003-023-05744-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Bar charts showing the amino acid usages at IMGT position 24 amongst natural λ-antibodies (plum) and natural κ-antibodies (seagreen). b Bar charts showing the amino acid usage at IMGT position 26 across natural λ-antibodies that are green-flagged for PSH, or that are red-flagged where position 26 is involved in the subset of most hydrophobic interactions. c, d Bar charts showing (c) the light V gene subgroup usages amongst natural λ-antibodies that are green-flagged or red-flagged for PSH, and (d) the IGLV2 gene usages amongst natural λ-antibodies that are green-flagged or red-flagged for PSH.