TABLE 3.
Accumulation of PHA by recombinant P. putida GPp104 harboring PHA biosynthesis genes of Pseudomonas sp. strain 61-3a
| Plasmid (relevant markers) | Substrate | Dry cell wt (g/liter) | PHA content (wt%) | PHA composition (mol%)b
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| 3HB (C4) | 3HHx (C6) | 3HO (C8) | 3HD (C10) | 3HDD (C12) | 3H5DD (C12′) | ||||
| pJHS60 (phbR phbB phbA phbC) | Gluconate | 0.89 | 20 | 100 | 0 | 0 | 0 | 0 | 0 |
| Octanoate | 0.87 | Trace | 100 | 0 | 0 | 0 | 0 | 0 | |
| Dodecanoate | 0.77 | 1 | 100 | 0 | 0 | 0 | 0 | 0 | |
| pJHS60dBA (phbR phbC) | Gluconate | 0.80 | 1 | 100 | 0 | 0 | 0 | 0 | 0 |
| pJHS48 (phbB phbA phbC) | Gluconate | 0.80 | 0 | ||||||
| pJASc60 (phaC1 phaZ phaC2 phaD) | Gluconate | 0.62 | 6 | 0 | 1 | 8 | 53 | 23 | 15 |
| Octanoate | 1.06 | 29 | 3 | 15 | 81 | 1 | 0 | 0 | |
| Dodecanoate | 0.96 | 21 | 5 | 13 | 37 | 29 | 16 | 0 | |
| Tetradecanoate | 0.89 | 23 | 4 | 11 | 37 | 31 | 17 | 0 | |
| pJASc22 (phaC1) | Gluconate | 0.88 | 9 | 0 | 2 | 15 | 58 | 14 | 11 |
| Octanoate | 1.17 | 43 | 3 | 16 | 77 | 2 | 0 | 0 | |
| Dodecanoate | 0.61 | 33 | 3 | 12 | 39 | 29 | 17 | 0 | |
| Tetradecanoate | 0.97 | 38 | 3 | 12 | 39 | 28 | 18 | 0 | |
| pJASc60dC1Z (phaC2 phaD) | Gluconate | 0.95 | 10 | 0 | 1 | 13 | 62 | 14 | 10 |
| Octanoate | 0.84 | 6 | 0 | 10 | 72 | 18 | 0 | 0 | |
| Dodecanoate | 0.64 | 5 | 0 | 7 | 39 | 29 | 25 | 0 | |
| Tetradecanoate | 0.61 | 5 | 0 | 6 | 33 | 34 | 27 | 0 | |
a
Cells were cultivated at 30°C for 72 h in MS medium containing the sodium salt of gluconate (2% wt/vol), octanoate, dodecanoate, or tetradecanoate (0.5% wt/vol) as the sole carbon source.
b
3HDD, 3-hydroxydodecanoate; 3H5DD, 3-hydroxy-cis-5-dodecenoate.