TABLE 4.
Accumulation of PHA by recombinant R. eutropha PHB−4 harboring PHA biosynthesis genes of Pseudomonas sp. strain 61-3a
| Plasmid (relevant markers) | Substrate | Dry cell wt (g/liter) | PHA content (wt%) | PHA composition (mol%)b
|
|||||
|---|---|---|---|---|---|---|---|---|---|
| 3HB (C4) | 3HHx (C6) | 3HO (C8) | 3HD (C10) | 3HDD (C12) | 3H5DD (C12′) | ||||
| pJHS60 (phbR phbB phbA phbC) | Gluconate | 1.62 | 47 | 100 | 0 | 0 | 0 | 0 | 0 |
| Octanoate | 1.06 | 40 | 100 | 0 | 0 | 0 | 0 | 0 | |
| Dodecanoate | 0.72 | 30 | 100 | 0 | 0 | 0 | 0 | 0 | |
| pJHS60dBA (phbR phbC) | Gluconate | 1.56 | 51 | 100 | 0 | 0 | 0 | 0 | 0 |
| Octanoate | 1.09 | 35 | 100 | 0 | 0 | 0 | 0 | 0 | |
| Dodecanoate | 0.61 | 26 | 100 | 0 | 0 | 0 | 0 | 0 | |
| pJHS48 (phbB phbA phbC) | Gluconate | 1.71 | 54 | 100 | 0 | 0 | 0 | 0 | 0 |
| Octanoate | 1.75 | 33 | 100 | 0 | 0 | 0 | 0 | 0 | |
| Dodecanoate | 0.51 | 14 | 100 | 0 | 0 | 0 | 0 | 0 | |
| pJASc60 (phaC1 phaZ phaC2 phaD) | Gluconate | 0.85 | 12 | 100 | 0 | 0 | 0 | 0 | 0 |
| Octanoate | 0.84 | 6 | 92 | 0 | 8 | 0 | 0 | 0 | |
| Dodecanoate | 0.30 | 6 | 100 | 0 | 0 | 0 | 0 | 0 | |
| Tetradecanoate | 0.69 | 4 | 91 | 0 | 3 | 3 | 3 | 0 | |
| pJASc22 (phaC1) | Gluconate | 0.74 | 2 | 100 | 0 | 0 | 0 | 0 | 0 |
| Octanoate | 0.86 | 13 | 31 | 10 | 59 | 0 | 0 | 0 | |
| Dodecanoate | 0.61 | 5 | 31 | 4 | 23 | 23 | 19 | 0 | |
| Tetradecanoate | 0.97 | 14 | 46 | 4 | 21 | 18 | 11 | 0 | |
| pJASc60dC1Z (phaC2 phaD) | Gluconate | 0.92 | 20 | 100 | 0 | 0 | 0 | 0 | 0 |
| Octanoate | 0.74 | 4 | 50 | 7 | 43 | 0 | 0 | 0 | |
| Dodecanoate | 0.34 | 1 | 51 | 0 | 9 | 13 | 27 | 0 | |
| Tetradecanoate | 0.67 | 5 | 44 | 1 | 16 | 15 | 24 | 0 | |
a
Cells were cultivated at 30°C for 72 h in MS medium containing the sodium salt of gluconate (2% wt/vol), octanoate (0.1% [wt/vol] × 5), dodecanoate, or tetradecanoate (0.5% wt/vol) as the sole carbon source.
b
3HDD, 3-hydroxydodecanoate; 3H5DD, 3-hydroxy-cis-5-dodecenoate.