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. 2024 Jan 8;108(1):80. doi: 10.1007/s00253-023-12956-9

Table 2.

Substrate specificity analysis of recombinant L. mucosae INIA P508 GluLm.

Substrate (1 mmol L−1) Relative activity (%)
p-Nitrophenyl β-D-glucopyranoside (pNPG) 100.0 ± 0.0a
p-Nitrophenyl α-D-glucopyranoside 2.2 ± 0.0a
p-Nitrophenyl β-D-galactopyranoside (pNPGAL) 68.8 ± 0.2a
p-Nitrophenyl α-D-galactopyranoside 0.3 ± 0.0a
p-Nitrophenyl β-D-fucopyranoside (pNPBFU) 95.2 ± 0.1a
p-Nitrophenyl α-L-fucopyranoside (pNPAFU) 26.6 ± 0.0a
p-Nitrophenyl β-D-xylopyranoside (pNPXYL) 96.8 ± 0.0a
p-Nitrophenyl α-D-xylopyranoside 0.1 ± 0.0a
Cellobiose 2.89 ± 0.41b
Daidzin 67.95 ± 1.58b
Genistin 64.13 ± 3.04b
Oleuropein 34.05 ± 3.73b
Quercetin-3-glucoside 11.13 ± 0.67b
Quercetin-3-rutinoside 1.40 ± 0.41b
Lactose 2.26 ± 1.30b
Trehalose 0.84 ± 0.74b
2’-Fucosyllactose 0.12 ± 0.09c
3-Fucosyllactose 0.00 ± 0.00c

aThe indicated activity is relative to the concentration of p-nitrophenol released

bThe indicated activity is relative to the concentration of glucose released

cThe indicated activity is relative to the concentration of fucose released