Table 2.
Substrate specificity analysis of recombinant L. mucosae INIA P508 GluLm.
| Substrate (1 mmol L−1) | Relative activity (%) |
|---|---|
| p-Nitrophenyl β-D-glucopyranoside (pNPG) | 100.0 ± 0.0a |
| p-Nitrophenyl α-D-glucopyranoside | 2.2 ± 0.0a |
| p-Nitrophenyl β-D-galactopyranoside (pNPGAL) | 68.8 ± 0.2a |
| p-Nitrophenyl α-D-galactopyranoside | 0.3 ± 0.0a |
| p-Nitrophenyl β-D-fucopyranoside (pNPBFU) | 95.2 ± 0.1a |
| p-Nitrophenyl α-L-fucopyranoside (pNPAFU) | 26.6 ± 0.0a |
| p-Nitrophenyl β-D-xylopyranoside (pNPXYL) | 96.8 ± 0.0a |
| p-Nitrophenyl α-D-xylopyranoside | 0.1 ± 0.0a |
| Cellobiose | 2.89 ± 0.41b |
| Daidzin | 67.95 ± 1.58b |
| Genistin | 64.13 ± 3.04b |
| Oleuropein | 34.05 ± 3.73b |
| Quercetin-3-glucoside | 11.13 ± 0.67b |
| Quercetin-3-rutinoside | 1.40 ± 0.41b |
| Lactose | 2.26 ± 1.30b |
| Trehalose | 0.84 ± 0.74b |
| 2’-Fucosyllactose | 0.12 ± 0.09c |
| 3-Fucosyllactose | 0.00 ± 0.00c |
aThe indicated activity is relative to the concentration of p-nitrophenol released
bThe indicated activity is relative to the concentration of glucose released
cThe indicated activity is relative to the concentration of fucose released