FIG. 1.

Spore outgrowth (A) and PG synthesis (B) in strains lacking HMW PBPs. The spores were heat shocked and inoculated into 2× YT medium with 4 mM l-alanine containing [³H]N-acetyl-d-glucosamine, as described in Materials and Methods. The cells were harvested, and TCA-insoluble radioactivity was measured as described in Materials and Methods. The percentage of total radioactivity in the sample that was TCA insoluble was divided by the initial OD₆₀₀ to give the units in Fig. 1B. The kinetics of spore outgrowth shown are from the same experiment in which PG synthesis was monitored. □, PS832 (wild type); ○, PS2465 (pbpA); ◊, PS2466 (pbpA ponA); ▵, PS2468 (pbpA pbpD).