FIG. 2.

PG turnover during outgrowth of wild-type and pbpA spores. The spores were heat shocked and germinated in 2× YT medium with 4 mM l-alanine for 30 min in the presence of [³H]N-acetyl-d-glucosamine. Then the cells were harvested and resuspended in fresh 2× YT medium with unlabeled N-acetyl-d-glucosamine, and TCA-insoluble radioactivity was measured, as described in Materials and Methods. The amount of TCA-insoluble radioactivity immediately after resuspension was set at 100%. □, OD₆₀₀ of PS832 (wild type); ○, OD₆₀₀ of PS2465 (pbpA); ■, percent incorporated [³H]N-acetyl-d-glucosamine remaining in PS832 (wild type); •, percent incorporated [³H]N-acetyl-d-glucosamine remaining in PS2465 (pbpA).