FIG. 5.

Morphologies of cells and outgrowing spores of the wild-type strain and strains lacking PBP2a or PBP2a and PBP1. The spores were heat shocked and inoculated into either PAB medium, PAB medium with 500 mM NaCl, or 2× YT medium, all of which contained 4 mM l-alanine. Outgrowing spores were harvested 120 min after the initiation of spore germination unless otherwise noted. The following strains (genotypes) and growth or outgrowth conditions are shown: (A) PS2062 (ponA), vegetative cells in PAB medium with 50 μM MgCl₂; (B) PS2466 (pbpA ponA), vegetative cells in PAB medium with 50 μM MgCl₂; (C) PS2466 (pbpA ponA), spores outgrowing in 2× YT medium with no additions, harvested 150 min after the initiation of spore germination; (D) PS832 (wild type), spores outgrowing in PAB medium with no additions; (E) PS2465 (pbpA), spores outgrowing in PAB medium with no additions; (F) PS2465 (pbpA ponA), spores outgrowing in PAB medium with no additions; and (G) PS2465 (pbpA), spores outgrowing in PAB medium with 500 mM NaCl. Bars, 10 μM.