TABLE 2.
Spore outgrowth phenotypes of HMW PBP mutantsa
| Strain | Morphology (outgrowth rateb) in:
|
||||
|---|---|---|---|---|---|
| PAB | PAB + 500 mM NaCl | PAB + 10 mM Mg2+ | 2× YTc | 2× YT + 10 mM Mg2+ | |
| PS832 (wtd) | wte (115) | Elongation, then lysis (150) | wt (107) | wt (105) | wt (103) |
| PS2465 (pbpA) | ↑f Cell widthe (>180) | Spherical, then lysise (150) | ↑ Cell width (108) | ↑ Cell width (108) | ↑ Cell width (110) |
| PS2466 (pbpA ponA) | ↓ Cell width; poor elongatione (>180) | No ↑ in OD | ↓ Cell width (125) | ↓ Cell width; bendinge (>150) | ↓ Cell width (123) |
| PS2468 (pbpA pbpD) | ↑ Cell width (>180) | ↑ Cell width; elongation, then lysis (150) | ↑ Cell width (111) | ↑ Cell width (140) | ↑ Cell width (113) |
a
Germination was done at 37°C with the inclusion of 4 mM l-Ala in the medium, as described in Materials and Methods. Spores in PAB medium were centrifuged 30 min after the initiation of spore germination, as described in Materials and Methods.
b
Values in parentheses are the minimum times in minutes needed for the initial OD600 of a culture to double after the addition of spores to the germination medium.
c
See Fig. 1A.
d
wt, wild type.
e
See Fig. 5.
f
↑, increased; ↓, decreased.