Table 1.
Primers and probes used in this study
| Primers | Sequences (5′–3′) | Description |
|---|---|---|
| TV-F1 | TCCATTAAGAAACCTCAAGAGATGACAAGAG | |
| TV-F2 | ATTGCTTATGAATTCGACATGGGATATTCC | |
| TV-F3 | ACCCCAAACTTGCAATGACACTCAAATTGC | |
| TV-R1 | TAATACGACTCACTATAGGGACTGAGGGTAAGACCAATGTTCGACAATAG | |
| TV-R2 | TAATACGACTCACTATAGGGTTGTTGTAGTTGTCAAGGACTGCCTTTGCG |
Candidates for MIRA primers screened in this study TV-F5/TV-R5 pair was used for follow-up experimental tests (this paper) |
| TV-R3 | TAATACGACTCACTATAGGGACCTTCACTTTGGATTTGACTTCGGAGAAG | |
| TV-F4 | TCGACATGGGATATTCCATTAAGAAACCTC | |
| TV-F5 | ACTTGCAATGACACTCAAATTGCTTATGAA | |
| TV-F6 | ACACCCCAAACTTGCAATGACACTCAAATTGCTTA | |
| TV-F7 | TGCAATGACACTCAAATTGCTTATGAATTCG | |
| TV-R4 | TAATACGACTCACTATAGGGTTGCGAACTGAGGGTAAGACCAATGTTCGACAATAGGT | |
| TV-R5 | TAATACGACTCACTATAGGGTACTAAGCCACTTTGACCTTGAGTGAATTCGTACTTC | |
| TV-R6 | TAATACGACTCACTATAGGGAATGTTCGACAATAGGTTTTAATGTGGTTACTAAGCCAC | |
| TV-R7 | TAATACGACTCACTATAGGGAGACCAATGTTCGACAATAG | |
| TV-crRNA | GAUUUAGACUACCCCAAAAACGAAGGGGACUAAAACUUAUGAGAAGGUCGACAACUUUGUUGAA |
crRNA (this paper) |
| 13U-FB-reporter | FAM-UUUUUUUUUUUUU-Bio | Reporter |
| 6U-FB-reporter | FAM-UUUUUU-Bio | |
| TV-8S | TCTGGAATGGCTGAAGAAGACG | Nested PCR primers [26] (actin gene) |
| TV-9R | CAGGGTACATCGTATTGGTC | |
| TV-10S | CAGACACTCGTTATCG | |
| TV-11R | CGGTGAACGATGGATG |
TV Trichomonas vaginalis, FB FAM (carboxyfluorescein) and biotin