Fig 2. PfCen1, 3 and HsCen2 undergo calcium-dependent and reversible LLPS in vitro.

(A) Widefield image of highly concentrated recombinant centrin solutions to promote high droplet density of PfCen1-6His (200 μM), PfCen2-6His (120 μM), PfCen3-6His (193 μM), PfCen4-6His (120 μM), HsCen2 (200 μM) before, after 2 mM CaCl₂ addition, and after 10 mM EDTA addition. Scale bars, 10 μm. Inlays show time lapse images of droplet fusion events. Movie metadata was used to estimate the time after calcium addition (bottom) (B) Turbidity measurements in centrin solutions at 30 μM (and higher concentration where indicated) during calcium addition followed by EDTA. Conditions: 50 mM BisTris (pH 7.1) at 37°C.