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. 2023 Dec 27;19(12):e1011899. doi: 10.1371/journal.ppat.1011899

Fig 3. Centrins from highly divergent eukaryotes can undergo phase separation in vitro.

Fig 3

(A) Turbidity measurements of recombinant T. brucei centrin A (TbCenA, 196 aa), C. reinhardtii centrin (CrCen, 169 aa) and S. cerevisiae centrin (Cdc31, 161 aa) at 30 μM (and higher concentrations where indicated) during calcium addition to 2 mM followed by 10 mM EDTA. Conditions: 50 mM BisTris (pH 7.1) at 37°C. (B) Using the IUpred 3.0 prediction we find a correlation between intrinsic disorder region probability (red line) and LLPS in vitro. (C) Widefield images of recombinant centrin solutions of TbCenA (100 μM), ScCdc31 (200 μM), CrCen (100 μM) after 2 mM CaCl2 addition show droplets and surface wetting, which dissolves quickly after addition of 10 mM EDTA. Scale bar, 10 μm.