Fig 4. PfCen1 and PfCen3 interact through co-condensation.

(A-C) Turbidity measurements of recombinant PfCen1-6His and PfCen3-6His at individually subcritical concentrations either independently or as a mixture during addition of calcium followed by EDTA. Individual centrins were supplemented with BSA to a consistent total protein concentration of >20 μM. (D) Brightfield and fluorescence imaging of preformed 30 μM PfCen3-6His protein droplets in presence of Ca²⁺ before and 30 s after addition of recombinant PfCen1-GFP-6His at 30 μM. Focus was adjusted between timepoints. Scale bar, 10 μm. All conditions: 50 mM BisTris (pH 7.1) at 37°C.