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. 2023 Dec 27;19(12):e1011899. doi: 10.1371/journal.ppat.1011899

Fig 6. 1,6-hexanediol treatment dissolves centrin foci in vivo.

Fig 6

(A) Turbidity measurements of recombinant PfCen1 solution at 30 μM in 50 mM BisTris (pH 7.1) at 37°C during addition of calcium followed by 5% 1,6-hexanediol and EDTA. (B) Immunofluorescence of non-overexpressing parasites treated with 5% 1,6-hexanediol or untreated control for 5 min stained with anti-PfCen1 and anti-Tubulin antibodies. Images are MIP. DNA stained with Hoechst. Scale bars; 1 μm. (C) Quantification of spindles in treated (n = 110) and control (n = 85) cells (early schizonts) with or without centrin focus at the spindle pole in percentage. Absolute numbers given in columns.