Fig. 2. rag^−/− mice do not exhibit a heightened proinflammatory cytokine expression at peak weight loss.

(A to C) Flow cytometry analysis of lung cells 3, 7, and 14 dpi. (A) Number of lung neutrophils (CD11b⁺, MHC-II⁻, and Ly6G⁺ cells). (B) Number of monocyte-derived macrophage (CD11b⁺, CD11c⁺, and F4/80⁺ cells). (C) Percentage of MHC-II⁺ monocyte-derived macrophage (CD11b⁺, CD11c⁺, F4/80⁺, and MHC-II⁺ cells) in the lungs of wild-type (WT) and rag^−/− mice at days 3, 7, and 14 after infection. WT control: n = 3, D3: n = 3, D7: n = 8, D14: n = 3; rag^−/− control: n = 3, D3: n = 7, D7: n = 7, D14: n = 5; the experiment was performed once for each time point. Two-way ANOVA with Tukey’s multiple comparisons test. (D to G) Cytokine concentrations from the bronchoalveolar lavage fluid at days 3 and 14 after infection. (D) IL-6, (E) TNF-α, (F) IFN-γ, and (G) IL-2. WT D3: n = 4, D14: n = 3; rag^−/− D3: n = 5, D14: n = 5, experiment performed once for each time point. Two-way ANOVA with Sidak’s multiple comparisons test. (H to M) mRNA levels from lung samples of infected animals at days 3 and 7 after infection. (H) Relative expression of IFN-α at 3 dpi WT: n = 7, rag^−/−: n = 12. (I) Relative expression of IFN-α at 7 dpi. WT: n = 7, rag^−/−: n = 7. (J) Relative expression of IFN-β at 3 dpi. WT: n = 7, rag^−/−: n = 12. (K) Relative expression of IFN-β at 7 dpi. WT: n = 7, rag^−/−: n = 7. (L) Relative expression of IFIT3 at 3 dpi. WT: n = 7, rag^−/−: n = 12. (M) Relative expression of IFIT3 at 7 dpi. WT: n = 7, rag^−/−: n = 7. (N) Relative expression of Rab27a 3 dpi. WT: n = 6, rag^−/−: n = 12. (O) Relative expression of Rab27a 7 dpi. WT: n = 7, rag^−/−: n = 7. (P) Relative expression of CNP 3 dpi. WT: n = 6, rag^−/−: n = 12. (Q) Relative expression of CNP 7 dpi. WT: n = 6, rag^−/−: n = 12. (H to M) *P < 0.05, **P < 0.01, two-tailed Mann-Whitney test.