FIG. 2.

Effect of growth temperature on the accumulation of VirB9 and VirB10. A. tumefaciens A348 was induced overnight in ABIM containing 200 μM AS at either 19 or 28°C, as indicated. Cells were washed three times in sodium phosphate buffer (pH 7.6), cross-linked with 200 μM BS³ (lanes 3 and 4) or DSS (lanes 5 and 6), and processed for electrophoresis on an SDS–7% (A) or –10% (B) polyacrylamide gel and immunoblotting as described in Materials and Methods. (A) Probed with anti-VirB10; (B) probed with anti-VirB9. The migration positions of proteins of known molecular mass (in kilodaltons) are shown at the right.