FIG. 4.

Accumulation of VirB proteins in wild-type and chvB mutant cells at 19 and 28°C. A. tumefaciens A348 (wild type) (lanes 1 and 3) and A1020 (chvB::Tn5) (lanes 2 and 4) were induced overnight at either 19 or 28°C, as indicated, in ABIM containing 200 μM AS. Cells were pelleted, and total crude extracts were subjected to electrophoresis on a 10% gel and immunoblot analysis as described in Materials and Methods. (A) Probed with anti-VirB10; (B) probed with anti-VirB11; (C) probed with anti-VirB9; (D) probed with anti-VirB8. The migration positions of proteins of known molecular mass (in kilodaltons) are shown at the left.