Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Dec 21;21(1):132–141. doi: 10.1038/s41592-023-02098-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 9 — (a) Images of a fluorescent slide at 1 mm depth using the Cousa, the Thorlabs 10x/0.5NA, and the Nikon 16x/0.8NA objectives with the laser beam scanning ±5° at the back aperture. The brightness of each image was normalized to the peak brightness, individually. Scan angles of ±5° correspond to a ~ 3 mm-wide field-of-view for the Cousa and Thorlabs 10x objectives, and a ~ 1.8 mm-wide field-of-view for the Nikon 16x objective. (b) The profiles of normalized brightness along the colored, dashed lines in (a). Note that the Cousa and the Thorlabs 10x/0.5NA have similar field uniformity, despite the Cousa being >12 mm further from the focal plane. The field brightness drops off rapidly from the center for the Nikon 16x/0.8NA objective.