Fig. 4.

Evaluation of cell viability in recellularized lungs. Recellularized lung scaffolds were evaluated via Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) staining and immunofluorescence (IF) staining for SPC in (a) Group A, (b) Group AM, and (c) Group M. (Scale bar = 50 μm). (d) Quantification of apoptotic cells of stained slides with Group A showing a significantly higher amount of TUNEL-positive apoptotic cells on Day 1. (e) Quantification of the proportion of TUNEL⁺SPC⁺ double-positive cells in the SPC⁺ AT2 cell population at Day 1 showing less apoptotic SPC⁺ in Group AM. Results are shown as group mean ± SD and analyzed via a Tukey HSD test, ∗:p < 0.05.