Skip to main content
. 2024 Jan 9;108(1):76. doi: 10.1007/s00253-023-12839-z

Table 1.

Primers used for the amplification of the vector and the inserts (genes encoding the modified endolysins). Bolded fragments in capitals indicate regions complementary to the vector sequence, and underlined fragments indicate regions complementary to the insert sequence. The sequence of the introduced linker is in italics. F and R indicate the orientation of the primers

Primers for insert (LysJ) GAAGGAGATATACATATGgacagaatattaatcattcccgat
TCAGTGGTGGTGGTGGTGGTGtcctccccccttcacatacacataggctt
Primers for insert (LysF) GAAGGAGATATACATATGgacagagtattgatcattcccgat
TCAGTGGTGGTGGTGGTGGTGtcctccccccttcacatacacataggctt
Primers for vector CATATGTATATCTCCTTCTTAAAGTTAAACAAAA
ggaCACCACCACCACCACCACTGA