Table 2.

The host range of phages J5a and F16Ba and the lytic spectrum of their modified endolysins, LysJ and LysF

Organism (number of strains)	Strain	ϕ J5a lysis	ϕ F16Ba lysis	Spot assay	Turbidity Reduction assay			References
				LysJ	LysF	LysJ	LysF
B. anthracis (6)	Sterne 34F21	+	+	+	+	+  +  +	+  +  +	(Dwyer et al. 2004; Negus et al. 2013)
	2112	+	+	+	-	+  +  +	+  +	(Szymajda and Bartoszcze 2005; Negus et al. 2013)
	1153	+	+	+	-	+  +  +	+  +  +	(Cieślik et al. 2015; Graniak et al. 2020)
	15832	+	+	+	-	+  +  +	+  +  +	(Szymajda and Bartoszcze 2005; Graniak et al. 2020)
	15842	n.a	n.a	+	-	+  +  +	+  +	(Szymajda and Bartoszcze 2005; Graniak et al. 2020)
	PZH4	+	+	+	+	+  +  +	+  +  +
B. cereus (11)	ATCC 10872	-	-	-	-	+	+
	ATCC 108763	-	-	-	-	+	+	(Negus et al. 2013)
	ATCC 11778	-	-	n.a	n.a	n.a	n.a
	ATCC 134723	-	-	-	-	+  +	+  +	(Negus et al. 2013)
	ATCC 14579 T 3	-	-	-	-	+	+	(Negus et al. 2013)
	ATCC 196373	-	-	-	-	-	-	(Negus et al. 2013)
	ATCC 232613	-	-	-	-	±	±	(Negus et al. 2013)
	F169593	-	-	-	-	-	-	(Dwyer et al. 2004)
	F172023	-	-	-	-	+  +  +	+  +  +	(Dwyer et al. 2004)
	F172893	-	-	-	-	+  +  +	+  +  +	(Dwyer et al. 2004)
	UW853	-	-	-	-	-	-	(Dwyer et al. 2004)
B. thuringiensis (11)	ATCC 33679	-	-	-	-	+  +	+  +	(Negus et al. 2013)
	ATCC 356463	-	-	-	-	+  +	+  +	(Dwyer et al. 2004)
	ATCC 10792	-	-	-	-	±	±	(Negus et al. 2013)
	ATCC 10792 T	-	-	-	-	+  +	+  +	(Dwyer et al. 2004)
	T07-0193	-	-	-	-	+  +	+  +	(Negus et al. 2013; Graniak et al. 2020)
	T07-1283	-	-	-	-	+  +	+  +	(Negus et al. 2013; Graniak et al. 2020)
	T07-1463	-	-	n.a	n.a	n.a	n.a	(Qi et al. 2001; Graniak et al. 2020)
	T07-1513	-	-	-	-	+  +	+  +	(Graniak et al. 2020)
	T07-1553	-	-	-	-	+  +	+  +	(Graniak et al. 2020)
	T07-2023	-	-	-	-	+  +  +	+  +  +	(Qi et al. 2001; Cieślik et al. 2015)
	#353	-	-	-	-	+  +	+  +	(Cieślik et al. 2015)
B. mycoides (3)	ATCC 6462	-	-	-	-	-	-
	ATCC 219293	-	-	-	-	+	+	(Dwyer et al. 2004)
	K1843	-	-	n.a	n.a	n.a	n.a	(Czaban et al. 2004)
B. sp. Ba 813 + (10)	#6 (I/2)3	-	-	-	-	+	+	(Negus et al. 2013; Graniak et al. 2020)
	#7 (II/3)3	-	-	-	-	+	+	(Graniak et al. 2020)
	#12 (S8553/2)3	-	-	-	-	+  +	+  +	(Dwyer et al. 2004; Graniak et al. 2020)
	#16 (PJ572)3	-	-	-	-	-	-	(Niemcewicz and Bartoszcze 2006a; Graniak et al. 2020)
	#17 (094)3	-	-	-	-	+	+	(Dwyer et al. 2004; Graniak et al. 2020)
	#21 (T1197-77)3	-	-	-	-	-	-	(Dwyer et al. 2004; Graniak et al. 2020)
	#28 (3)3	-	-	-	-	+  +  +	+  +  +	(Niemcewicz and Bartoszcze 2006a; Graniak et al. 2020)
	#30 (1B)3	-	-	-	-	+  +	+  +	(Graniak et al. 2020)
	#313	-	-	-	-	+	+	(Graniak et al. 2020)
	#34033	-	-	-	-	+	+  +	(Dwyer et al. 2004)
B. subtilis	ATCC 66333	-	-	-	-	+  +	+  +	(Negus et al. 2013)

In the columns with phage lytic activity and the results of spot tests the “ + ” or “- “ signs indicate the presence or lack of lysis zones, respectively. In the column with the results of the turbidity reduction assay, the “ + ” sign indicates the degree of the optical density reduction: slight ( +), moderate (+ +), or intense (+ + +)

n.a. non-applicable

Notes: ¹Strain isolated from “Antraphyl” vaccine by Phylaxia-Sanofi (serial no. 021OE2)

²Strain acquired by the Military Institute of Hygiene and Epidemiology (MIHE) from the Institute of Veterinary Hygiene in Białystok, the field office in Łomża, Poland

³Strains acquired by MIHE from the Institute of Molecular Biology and Medicine (IMBM) at the University of Scranton, PA, USA

⁴Strain acquired by MIHE from the National Institute of Public Health – National Institute of Hygiene, Poland