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. 2024 Jan 9;15:386. doi: 10.1038/s41467-023-44669-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — a Experimental scheme for assessing S. aureus-induced pulmonary inflammation and associated lung injury in WT and neutrophil-specific Gsdme conditional KO mice. Pneumonia was induced in neutrophil-specific Gsdme conditional KO (Mrp8-cre(+)/Gsdme^Δ/Δ) and their WT (Mrp8-cre(-)/Gsdme^fl/fl) littermates using S. aureus. Neutrophil recruitment, pulmonary inflammation, and lung damage were evaluated at different time points post-infection. b Quantification of total leukocytes and neutrophils in the BALF from Mrp8-cre(-)/Gsdme^fl/fl and Mrp8-cre(+)/Gsdme^Δ/Δ mice post-pneumonia induction at indicated time points. Data are represented as mean ± SEM (n = 4–6 mice /group). *P < 0.05, **P < 0.01 by two-sided Student’s t-test (WBC: 24 h, P = 0.0115; 48 h, P = 0.0125. Neutrophils: 24 h, P = 0.0107; 48 h, P = 0.0058). c IL-1β, IL-1, and IL-10 levels in the BALF from mice infected with S. aureus. Data are shown as mean ± SEM (n = 4–6 mice /group). *P < 0.05. ND not detectable (IL-10, P = 0.0221. IL-1β: 24 h, P = 0.0118; 48 h, P = 0.0140. IL-6: 24 h, P = 0.0081; 48 h, P = 0.0435). d LDH release in BALF post-infection. Data are represented as mean ± SEM (n = 4–6 mice /group). *P < 0.05 by two-sided Student’s t-test (24 h, P = 0.0049; 48 h, P = 0.0215). e Total protein concentration in the BALF. Data are shown as mean ± SEM (n = 4–6 mice/group). *P < 0.05 by two-sided Student’s t-test (24 h, P = 0.009; 48 h, P = 0.0453). f Bacterial burden in the lungs of Mrp8-cre(-)/Gsdme^fl/fl and Mrp8-cre(+)/Gsdme^Δ/Δ mice post S. aureus infection at the specified time points. Data are represented as mean ± SEM (n = 4–6 mice/group). **P < 0.01. NS not significant by two-sided Student’s t-test (P = 0.0035). g Bacterial count in the BALF from Mrp8-cre(-)/Gsdme^fl/fl and Mrp8-cre(+)/Gsdme^Δ/Δ mice post S. aureus infection at the specified time points. Data are presented as mean ± SEM (n = 4–6/ mice group). ***P < 0.001. NS not significant by two-sided Student’s t-test (P = 0.0007). Source data are provided as a Source Data file.