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. 2024 Jan 9;15:386. doi: 10.1038/s41467-023-44669-y

Fig. 9. GSDME disruption in neutrophils alleviated lung injury during S. aureus-induced pneumonia.

Fig. 9

a Hematoxylin and eosin (H&E) stained lung sections from Mrp8-cre(-)/Gsdmefl/fl and Mrp8-cre(+)/GsdmeΔ/Δ mice pre and post S. aureus-induced pneumonia. The scale bar indicates 100 μm. Results represent a minimum of three biological replicates. b Assessment of pulmonary inflammation and damage via neutrophil recruitment (vol % of alveoli) and edema in the lung area. Data are represented as mean ± SEM (n = 4 mice/group). **P < 0.01 by two-sided Student’s t-test. The data represents at least three independent experiments (Recruited neutrophils, P = 0.0074; Edema area of total area, P = 0.0055). c Survival analysis of WT and neutrophil-specific Gsdme conditional KO post S. aureus infection. Mrp8-cre(-)/Gsdmefl/fl and Mrp8-cre(+)/GsdmeΔ/Δ mice (n = 8–9 mice/group) were intratracheally infected with S. aureus (1.5 × 108 CFUs/mouse) and monitored for survival over 7 days. Data were analyzed using the Gehan-Breslow-Wilcoxon test. *P < 0.05 (P = 0.0383). Source data are provided as a Source Data file.