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. 2024 Jan 9;14:864. doi: 10.1038/s41598-023-51063-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Perivascular invasion and displacement of astrocyte end-feet by glioma cells. (a) U3013MG and U3054MG glioma cells in perivascular space (between blood vessel and astrocyte end-feet). Coronal sections were stained with HuNu (red, human glioma cells), AQP4 (white, astrocyte end-feet) and CD31 (green, blood vessels) antibodies and z-stacks of selected regions were taken by Leica confocal microscope using 63 × objective. Scale bar, 20 µm. (b) Interaction of human glioma cells (STEM121, red) with astrocyte end-feet and blood vessels. Each image represents a snapshot of a 3D image. Scale bar, 10 µm.