Figure 3.
In vitro chromatin assembly of A. thaliana chromatin combinations. The supercoiling assay results of the reconstituted twelve nucleosome combinations were analyzed on a 0.8% 0.5xTBE agarose gel and visualized by EtBr. The first lane contains untreated, supercoiled pBSK plasmid, which serves as a reference plasmid DNA topology. The second lanes contain deproteinized pBSK plasmid from reaction mixtures that only contained (H3.1-H4)2, (H3.1-H4)2, or (CENH3-H4)2 tetrasome combinations. The chromatin combinations with four core histones are represented in the rest of the lanes. The SC and RC indicate the positions of the supercoiled and relaxed circular plasmid, respectively, estimated from the untreated, supercoiled pBSK plasmid.