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. 2024 Jan 10;14:936. doi: 10.1038/s41598-024-51460-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of ISWI remodeling complex on seven reconstituted chromatin combinations. (a) The cell-free synthesized CHR11 and DDR4 proteins are shown on a 10% SDS-PAGE, stained by CBB. (b) The partial MNase digestion assay of the seven chromatin combinations (NCP, H2A.X NCP, H2A.W NCP, H3.3 NCP, H2A.X/H3.3 NCP, CENH3 NCP, and H2A.X/CENH3 NCP) reacted in the absence of remodeling factors, the presence of CHR11 mRNA or the presence of both CHR11 and DDR4 mRNAs were analyzed on a 2% agarose gel. Uncropped agarose gel images and technical replicates for (b) are available in Supplementary Information (S4–S6). (c) Nucleosome spacing indexes (NSIs) of each combination were estimated from the agarose gel densitometry results of MNase assay (n = 3), and mean NSI values were plotted on a bar graph. The jitter plots over the bar graphs show the individual values of three replicas, the individual graphs of each replicate can be found in the Supplementary Information (S9). Error bars represent the standard deviation from the three independent experiments. Asterisks denote the statistical significance of NSI values between samples: *p value < 0.05; **p value < 0.01; ***p value < 0.001; non-significant results were not plotted.