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. 2023 Dec 20;99:104922. doi: 10.1016/j.ebiom.2023.104922

Fig. 5.

Fig. 5

Dengue dextramer+ CD8+ T cells. CD8+ responses were evaluated using eight dengue dextramers HLA-A∗02, B∗07, and A∗03 in groups vehicle-GNP (n = 3), LD PepGNP-Dengue (n = 8), and HD PepGNP-Dengue (n = 8) at day 0, 35 and 180. (a) Number of dengue dextramer+ CD8+ T cells over 105 total CD8+ T cells, as the sum of dengue dextramer responses for each participant. Responses to one to seven dengue dextramers per participant were assessed according to their HLA expression and summed. Number of responders are indicated at the top of the panel. (b) Change from baseline as the ratio of post-/pre-vaccination response. (c) Memory phenotype of D-dextramer+CD8+ in vaccinees (LD and HD, n = 16, ◊). Subsets defined as naïve (CD45RA+CCR7+CD95−), Tscm (CD45RA+CCR7+CD95+), Tcm (CD45RA−CCR7+), Tem (CD45RA−CCR7−) and TemRA (CD45RA+CCR7−). Additionally, the functionality of dextramer+ CD8+ T cells was assessed by the expression of CXCR3. Intra-group comparisons with day 0 using Friedman tests and inter-group comparisons used Kruskal–Wallis tests followed with Dunn’s post tests. p values <0.05 are indicated. Bars indicate medians and 95% confidence intervals. Grey zones indicate cut-off responses (63.4 dextramer+CD8+/105 CD8+ T cells and a ratio of 1.54). LD = low dose (○); HD = high dose (▵); GNP = gold nanoparticles.