Table 3.
Dengue-specific respondersc in AIM, intention-to-treat analysis.
| Vehicle-GNP (n = 6) |
LD PepGNP-Dengue (n = 10) |
HD PepGNP-Dengue (n = 10) |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Day | 21 | 35 | 90 | 180 | Anya | 21 | 35 | 90 | 180 | Anya | 21 | 35 | 90 | 180 | Anya |
| CD8+CD107a+CD25+ | 1 (17%) | 0 (0%) | 0 (0%) | 1 (17%) | 2 (33%) | 1 (10%) | 1 (10%) | 2 (20%) | 1 (10%) | 3 (30%) | 1 (10%) | 2 (20%) | 1 (10%) | 0 (0%) | 4 (40%) |
| CD8+CD137+CD69+ | 1 (17%) | 0 (0%) | 0 (0%) | 1 (17%) | 2 (33%) | 5b(50%) | 2 (20%) | 3 (30%) | 3 (30%) | 6 (60%) | 0 (0%) | 1 (10%) | 4 (40%) | 3 (30%) | 4 (40%) |
| At least one co-marker | 1 (17%) | 0 (0%) | 0 (0%) | 1 (17%) | 2 (33%) | 2 (20%) | 1 (10%) | 2 (20%) | 3 (30%) | 6 (60%) | 0 (0%) | 0 (0%) | 1 (10%) | 0 (0%) | 1 (10%) |
| Any marker+ | 1 (17%) | 0 (0%) | 0 (0%) | 1 (17%) | 2 (33%) | 6 (60%) | 3 (30%) | 3 (30%) | 4 (40%) | 7 (70%) | 1 (10%) | 3 (30%) | 4 (40%) | 3 (30%) | 6 (60%) |
Number of responders per group is indicated for each dengue-specific CD8+ T cell parameter measured by AIM assay upon stimulation with dengue peptides or PepGNP-Dengue.
LD = low dose; HD = high dose; GNP = gold nanoparticles; AIM = activation-induced markers.
a
At any time post-vaccination. Comparison between groups was performed using Fisher’s tests.
b
p = 0.033, comparison between LD and HD.
c
Volunteer with a positive delta (post- minus pre-vaccination) response and a CD8+ T cell response above the mean + 2 SD of the dengue-specific CD8+ T cell response in all volunteers at day 0.