TABLE 3.
Effects of GlnK and GlnKY51N on NifL activity in different backgrounds
| Straina | Relevant genotype | Expression of nifH′-′lacZb (U/ml/OD600)
|
|
|---|---|---|---|
| −N | +N | ||
| NCM1528 | Wild type | 3,580 | <10 |
| NCM1974 | glnK | <10 | <10 |
| NCM1851 | ntrC | <10 | <10 |
| NCM1687 | glnD | 20 | <10 |
| NCM2086 | Wild type/Ptet-glnK+ | 3,090 | 1,060 |
| NCM2087 | glnK/Ptet-glnK+ | 3,860 | 1,240 |
| NCM2088 | ntrC/Ptet-glnK+ | 3,510 | 1,850 |
| NCM2093 | glnD/Ptet-glnK+ | 3,940 | 1,340 |
| NCM2089 | Wild type/Ptet-glnK5 (GlnKY51N) | 1,450 | 1,330 |
| NCM2090 | glnK/Ptet-glnK5 (GlnKY51N) | 2,200 | 910 |
| NCM2091 | ntrC/Ptet-glnK5 (GlnKY51N) | 2,990 | 1,450 |
| NCM2094 | glnD/Ptet-glnK5 (GlnKY51N) | 3,390 | 2,670 |
a
All strains carry a single chromosomal copy of a nifH′-′lacZ fusion at the trp locus and plasmid pNH3 (Ptac-nifLA).
b
See footnote b to Table 2. Cultures were grown in K medium under anaerobic conditions with 2 mM glutamine (−N) or with 2 mM glutamine plus 4 mM NH4Cl (+N).