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Northern blot analysis of stress induction of yvyD transcription in a relA mutant. Total RNA was isolated from B. subtilis BR16 and BR17 before (C, control) and after depletion of amino acids (AT0, transient phase; AS, 30 min after entry into stationary phase). A total of 2.5 μg of RNA was applied in each lane. The locations of RNA molecular size markers and the size of the yvyD transcript are marked. Note that induction of yvyD transcription does not occur in the relA mutant strain.
