Table 1.
The effect of biomaterials on hDPSC neural differentiation
|
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Reference | Authors | Year of publication | Cell source | Type of study | Biomaterial | Impact on differentiation | Differentiation review technique | Neuronal expression marker check | Resultant neuron type | |
| Scaffold | Growth factors/ cultures | |||||||||
|
| ||||||||||
| (14) | Pineda et al. | 2022 | hDPSC | In vitro | Nanopatternedpoly(lactide-co-caprolactone) (PLCL) scaffolds | Plastic-adherent monolayer cultures, floating dentosphere cultures | Indirect | ICCFlow Cytometry | Neural stem cell marker Nestin, glial markers GFAP, and S100ß, and neuronal markers [NeuN anddoublecortin (DCX)] | Not mentioned |
| (3) | Gao et al. | 2022 | hDPSC | In vitro | TPS-PN plates (poly-N-isopropylacrylamide-co-butyl acrylate coated TPS plates) | rVT, LN, PLO, PBS, PS, DPBS, Trypsin-EDTA, α-MEM, FBS, neurobasal-A, bFGF, EGF, B27, PN | Indirect | Flow cytometry,immunostaining | βIII-tubulin,nestin | Not mentioned |
| (18) | Drewry et al. | 2022 | Excluded (due to not being accessible) | --------- | ------------------- | ---------------------- | ----------- | ----------------- | ----------------- | ----------------- |
| (7) | Zheng et al. | 2021 | hDPSC | In vitro | Chitosan porous scaffolds | Low glucose DMEM, FBS, penicillin, streptomycin | Indirect | Western blot,IF staining,RT-PCR | GFAP, S100β, β-tubulin III | Not mentioned |
| (16) | Luo et al. | 2021 | hDPSC | In vitro/ in vivo Sprague-Dawley male rats | GelMA-bFGF hydrogels | Indirect | Immunohistochemical analysis, Western blot | GFAP, β-tubulin III | Not mentioned | |
| (19) | Farhang et al. | 2021 | hDPSC | In vitro | 3D hanging drop technique | Neurobasal media and 10% foetalcalf serum containing 20 ng/ml EGF, 40 ng/ml human bFGF (hbFGF), SHH protein | Indirect | ICC | β-tubulin III andNeuN | Not mentioned |
| (8) | Laudani et al. | 2020 | hDPSC | In vitro | Decellularized ECM scaffold | FBS, penicillin,streptomycin, and fungizone, trypsin/EDTA, a-MEM, ascorbic acid | Indirect | Cytofluorimetric, IF, SEM, RT-PCR | MAP2, β-III tubulin, neurofilament-heavy (NF-H), VIM, NF-L, PAX6 | Not mentioned |
| (17) | Hsiao et al. | 2020 | hDPSC | In vitro | 3DP-PLAS | ---------------------- | Indirect | IF staining | GFAP, nestin,neurofilament-M (NF-M), β-III tubulin, MAP2 | Neural precursor cells,neurogenic structures, astrocyte-like cells |
| (20) | Rafiee et al. | 2020 | hDPSC | In vitro | Neurosphere technique | 20 ng/ml EGF, 20 ng/ml bFGF, and 10 mg/ml heparin/ high-glucose DMEM with 10% FBS | Indirect | Quantitative polymerase chain reaction (qPCR), Western blot, and IF, Immunostaining, Western blot | MAP2, and neurogenin 1 (Ngn1) | Neural progenitor cells |
| (21) | Goudarzi et al. | 2020 | hDPSC | In vitro | Neurosphere technique | 5% E-CSF | Indirect | ICC, RT-PCR | Nestin, MAP2, Oct4, Sox2, NF-M | Not mentioned |
| (2) | Kang et al. | 2019 | hDPSC | In vitro | ------------------- | Protocol 1: BME and nerve growthfactor (NGF)Protocol 2: D609Protocol 3: bFGF, SHH, and RA | Indirect | Flow cytometry, cell cycle analyses, RT-qPCR, ICC, analysis of acetylcholine (Ach) secretion in culturemedia | ChAT, HB9, ISL1, BETA-3, MAP2 | Cholinergic neuronal-like cells |
| (5) | Pisciotta et al. | 2018 | hDPSC | In vitro | 3D floating sphereculture system | EGF, bFGF, DMEM/F12 culture medium,L-glutamine penicillin,streptomycin, B27 | Indirect | IF analysis, Western blot, pseudocolour analysis | Nestin,CD271, SOX-10, β-III tubulin, MAP2 | Neural progenitor cells, neural crest stemcells |
| (6) | Bojnordi et al. | 2018 | hDPSC | In vitro | Neurosphere technique | bFGF, EGF, B27, N2, poly-L-lysine-coated coverslips | Indirect | Flow cytometry, IF staining | Nestin, NF68,MAP2, β-tubulin | Neuroprogenitor cells, mature neural cells |
| (12) | Ghasemi Hamidabadi et al. | 2017 | hDPSC | In vitro | Chitosan-intercalated montmorillonite/PVAnanofibers | Serum-free DMEM/F12 with 2% B27 supplemented with 20 ng/mL bFGF and 20 ng/mL EGF | Indirect | RT-PCR,immunostaining | Oct-4, Nestin,NF-M, NF-H, MAP2, β-III tubulin | Neuron-like cells |
| (22) | Geng et al. | 2017 | hDPSC | In vitro | -------------------- | Resveratrol, DMEM/F12, 10 ng/ml bFGF, 500 μM BME and 10%FBS | Indirect | ICC staining, Western blot, RT-PCR | Nestin, Musashi and NF-M | Neuroprogenitor cells |
| (13) | Zhang et al. | 2016 | hDPSC | In vitro | Chitosan porous scaffolds | DMEM,FBS, penicillin,streptomycin | Indirect | RT-PCR, Western blot, IF assays | CNPase, MAP2, GFAP | Oligodendrocytes, astrocytes, neural cells |
| (23) | Martens et al. | 2014 | hDPSC | In vitro | Collagen hydrogel | Cell suspension, MEM,type I rat tail, sodium hydroxide/alpha modification(alpha-MEM) FBS,glutamine,penicillin, streptomycin, BME, trans-RA, forskolin, bFGF, platelet-derivedgrowth factor AA (PDGFaa), heregulin-beta-1(NRG) | Indirect | ICC, ELISA | laminin, p75, GFAP, CD104, nestin | Schwann cell-like phenotype |
| (15) | Feng et al. | 2014 | hDPSC | In vitro | 3D highly porous CS | Neural induction medium (NIM)-DMEM/F-12 medium, B27, N2, BDNF,NGF, bFGF | Indirect | RT-PCR, Western blot, IF | Nestin, CNPase, MAP2, GFAP | MAP-2+ neural cells, GFAP+ astrocytes and CNP+ oligodendrocytes |
|
| ||||||||||
hDPSC; Human dental pulp stem cell, FBS; Foetal bovine serum, DMEM; Dulbecco’s Modified Eagle medium, bFGF; Basic fibroblast growth factor, EGF; Epidermal growth factor, BME; β-mercaptoethanol, SHH; Sonic hedgehog, RA; Trans-retinoic acid, RT-PCR; Reverse transcription-polymerase chain reaction, IF; Immunofluorescence, ICC; Immunocytochemistry, GFAP; Glial fibrillary acidic protein, MAP2; Microtubule-associated protein 2, CS; Chitosan scaffold, PVA; Poly (vinyl alcohol), and D609; Tricyclodecane-9-yl-xanthogenate.