. 2023 Dec 6;10(1):e23336. doi: 10.1016/j.heliyon.2023.e23336

Table 1.

Real-time PCR primers.

Gene	Genbank Accession	Primer Sequences(5′to3′)	Size(bp)
Mouse GAPDH	GU214026.1	GAAGGTCGGTGTGAACGGATTTG CATGTAGACCATGTAGTTGAGGTCA	127
Mouse SIRT-1	NM_019812.3	GGGAACCTTTGCCTCATCTACATT	90
Mouse SIRT-1	NM_019812.3	CACCACCTAGCCTATGACACA	90
Mouse SREBP-1c	NM_011480.3	CCCGGCTATTCCGTGAACAT	111
Mouse SREBP-1c	NM_011480.3	GCAGATATCCAAGGGCATCTGA	111
mmu-miR-22-3p-RT	MIMAT0000531	GTCGTATCCAGTGCAGGGTCCGAG	60
mmu-miR-22-3p-RT	MIMAT0000531	GTATTCGCACTGGATACGACACAGTT
mmu-miR-22-3p-F	MIMAT0000531	GCGAAGCTGCCAGTTGAAG
mmu-miR-138-5p-RT	MIMAT0000150	GTCGTATCCAGTGCAGGGTCCGAGG TATTCGCACTGGATACGACCGGCCT	61
mmu-miR-138-5p-F	MIMAT0000150	GCGAGCTGGTGTTGTGAATC	61
U6	M10329.1	GTCGTATCCAGTGCAGGGTCCGAGG TATTCGCACTGGATACGACGTCATCC	107
U6–F	M10329.1	CGCTTCGGCAGCACATATACTAA	107
Universal reverse primer（micro-R）	/	AGTGCAGGGTCCGAGGTATT	/