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. 2023 Dec 18;51(2):26. doi: 10.3892/or.2023.8685

Figure 1.

Figure 1.

Rab25 suppresses colon cancer cell invasion. Cancer cells were transfected with the indicated vectors for 24 h. (A) Invasion was analyzed by using a 48-well chemotaxis chamber (**P<0.01 and ***P<0.001 vs. control vector). (B) Wound healing analysis for detecting cell migration (*P<0.05 vs. control vector). (C) 3D Matrigel invasion analysis was performed using 3D Matrigel-coated Transwell chambers for 5 days. Original magnification, ×100; scale bar, 50 µm. The distance between the invaded HCT-116 cells was measured in five different positions and then calculated (***P<0.001 vs. control vector). (D) The 3D Matrigel culture was analyzed for morphology and colony size of transfected HCT-116 cells with the indicated vectors on Matrigel for 5 days. Original magnification, ×100; scale bar, 200 µm. (E) Cancer cells were transfected with the indicated vectors or siRNAs for 24 h and invasion was analyzed by using a 48-well chemotaxis chamber (**P<0.01 vs. scrambled siRNA, ***P<0.001 vs. control vector). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; si, short interfering.