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. 2023 Dec 18;51(2):26. doi: 10.3892/or.2023.8685

Figure 4.

Figure 4.

Rab25 induces claudin-7 expression. Cancer cells were transfected with the indicated vectors for 24 h. (A) The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) Claudin-7 expression was visualized using immunofluorescence. Original magnification, ×400; scale bar, 20 µm. (C) Reverse transcription-quantitative PCR (***P<0.001 vs. control vector). (D and E) HCT-116 cells were transfected with the indicated vector for 24 h, serum-starved and treated with (D) CHX or (E) MG132 for 6 h. The cell lysates were analyzed by immunoblotting with the indicated antibodies (*P<0.05 vs. control vector, #P<0.05 vs. Rab25 overexpression). (F) HCT-116 cells were transfected with the indicated vector, serum-starved and treated with CQ for 12 h. The cell lysates were analyzed by immunoblotting with the indicated antibodies. Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; CHX, cycloheximide; CQ, chloroquine.