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. 2023 Dec 18;51(2):26. doi: 10.3892/or.2023.8685

Figure 5.

Figure 5.

Claudin-7 mediates Rab-25-suppressed cancer cell invasiveness. (A) Cancer cells were transfected with the indicated vectors for 24 h. The cell lysates were analyzed by immunoblotting with the indicated antibodies. (B) HCT-116 cells were transfected with the indicated vectors for 24 h. Invasion was analyzed using a 48-well chemotaxis chamber (*P<0.05 vs. control vector and ###P<0.001 vs. claudin-7 overexpression). The cell lysates were analyzed by immunoblotting with the indicated antibodies. The cancer cells were transfected with the indicated vector and siRNA for 24 h. (C) Invasion and (D) migration were analyzed using a 48-well chemotaxis chamber (*P<0.05 vs. control vector, **P<0.01 vs. control vector, #P<0.05 vs. Rab25 overexpression with scrambled siRNA, ##P<0.01 vs. Rab25 overexpression with scrambled siRNA and ###P<0.001 vs. Rab25 overexpression with scrambled siRNA). (E) HCT-116 cells were transfected with the indicated vector and siRNA for 24 h. The cell lysates were analyzed by immunoblotting with the indicated antibodies. (F) Ras activity was analyzed using the Ras-GTP pull down assay. Total protein expressions were analyzed by immunoblotting with the indicated antibodies. (G) HCT-116 cells were co-transfected with the indicated vectors for 24 h. Invasion was analyzed using a 48-well chemotaxis chamber (**P<0.01 vs. control vector, ###P<0.001 vs. claudin-7 overexpression). Representative results of at least three independent experiments with similar results. Rab25, Ras-related protein 25; si, short interfering.