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Siderophore nutrition tests. KDF541 (A) grown in Luria-Bertani broth and harboring plasmids pITS449 (EcoFepA) (C), pENB5 (StyFepA) (D), pTY994 (StyIroN) (E), or pCD3 (PaeFepA) (F) was plated in nutrient agar containing ampicillin (10 μg/ml), and 10 μl of 50 μM FeEnt was applied to a sterile paper disc on the agar surface. BN1071 (chromosomal EcoFepA) (B) was tested under the same conditions. A 1-cm ruler was embedded in the photograph.
