Table 1.
Details regarding antibody name, catalogue number, clone, concentration, and company from which it was obtained are provided for antibodies used in the study. Antibodies were knockout verified, are widely published on, and have been certifiably verified by the vendor
| Antibody | Company | Catalogue Number | Clone | Concentration for Immunofluorescence | Concentration for Western Blot |
|---|---|---|---|---|---|
| GFAP(AF488) | Invitrogen | 53–9892-82 | GA5 | 10 μg/mL | |
| GLUT-1 | Invitrogen | PA5-16,793 | Poly | 1:100 | |
| CD71 | Invitrogen | 14–0719-82 | OKT9 | 5 μg/mL | |
| PECAM(FITC) | Invitrogen | 11–0311-82 | 390 | 5 μg/mL | |
| VE-cadherin | Invitrogen | 14–1449-82 | 16B1 | 5 μg/mL | |
| Zo-1 | Invitrogen | 61–7300 | Poly | 10 μg/mL | 1 μg/mL |
| Occludin | Invitrogen | 71–1500 | Poly | 4 μg/mL | 0.5 μg/mL |
| Claudin 5 | Invitrogen | 35–2500 | 4C3C2 | 1:25 | 1:1000 |
| OAT1 | Invitrogen | PA5-26,244 | Poly | 1:100 | 1:1000 |
| OAT3 | Invitrogen | PA5-76,143 | Poly | 1:100 | 1:1000 |
| ENT1 | Proteintech | 11,337–1-AP | Poly | 1:100 | |
| ENT1 | Invitrogen | PA5-116,461 | Poly | 1:500 | |
| OATP1A2 | Invitrogen | PA5-42,445 | Poly | 1:100 | 1:1000 |
| OATP2A1 | Invitrogen | PA5-98,789 | Poly | 1:200 | 1:1000 |
| BCRP | Novus | NBP2-22,124 | 3G8 | 1:200 | 1:2000 |
| P-gp | Invitrogen | PA5-61,300 | Poly | 2 μg/mL | |
| P-gp | Abcam | ab261736 | Poly | 0.5 μg/mL | |
| MRP1 | Abcam | ab24102 | MRPm5 | 1:50 | 1:200 |
| MRP4 | Cell Signaling | 12,705 | D2Q2O | 1:200 | 1:1000 |
| MRP5 | Invitrogen | PA5-18,965 | Poly | 10 μg/mL | |
| MRP5 | Abcam | Ab180724 | Poly | 1:1000 | |
| CYP3A4 | Invitrogen | MA5-17,064 | 3H8 | 1:200 | 1:1000 |
| PXR | Invitrogen | PA5-72,551 | Poly | 1:50 | 1:1000 |
| CAR | R&D Systems | PP-N4111-00 | N4111 | 10 μg/mL | 2 μg/mL |
| AK1 | Invitrogen | PA5-52,297 | Poly | 1 μg/mL | 0.1 μg/mL |
| AK2 | Proteintech | 11,014–1-AP | Poly | 1:200 | 1:1000 |
| AK5 | Invitrogen | PA5-53,933 | Poly | 1.2 μg/mL | 0.09 μg/mL |
| AK6 | Novus | NBP2-67,116 | B1-F4 | 1:100 | 1:2000 |
| Β-Actin (HRP) | Cell Signaling | 12,262 | 8H10D10 | 1:5000 | |
| Anti-rabbit (HRP) | Abcam | ab97051 | Poly | 1:2000 | |
| Anti-mouse (HRP) | Abcam | ab97023 | Poly | 1:2000 | |
| Anti-rabbit (AF488) | Invitrogen | A-11008 | Poly | 1 μg/mL | |
| Anti-mouse (AF488) | Invitrogen | A-11001 | Poly | 0.67 μg/mL | |
| Anti-goat (AF488) | Invitrogen | A-11078 | Poly | 1 μg/mL |