Fig. 5.

CAFs stimulation induces activation of adhesion-related signaling pathways in CLDN18.2-expressing gastric cancer cells. a. RNA sequencing data were generated from CLDN18.2-overexpressing AGS cells co-cultured with or without CAFs. Genes with a P value < 0.05 and | log₂ (fold change) | > 1 were considered significantly differentially expressed. The top 50 upregulated and top 50 downregulated genes were selected for heatmap analysis. b. Volcano plot analysis of all up- and downregulated genes was performed. c-d. GO and KEGG analyses were performed to analyze the differential gene expression. e. Protein interaction analysis of differential genes was conducted